Solid Phase Extraction-Spectrophotometric Determination Of Isoxsuprine Hydrochloride In Different Samples Using Modified Magnetic Nanoparticales

A green, simple and sensitive spectrophotometric method was proposed for the determination of isoxsuprine hydrochloride (ISX), based on preconcentration with cetyltrimethylammonium bromide (CTAB) modified magnetic iron oxide nanoparticales (MNPs). ISX coupled diazotized metoclopramide drug via diazotization-coupling reaction in alkaline medium to form yellow-orange color product which extracted measured spectrophotometrically at λmax444 nm. Various parameters affecting azo-dye removal by adsorption (Fe3O4@CTAB) such as pH solution, volume reagent, surfactant, amount adsorbent type desorbing solvent were studied. The linear calibration graph obtained concentration range 0.3-7.0 µg/mL ISX, limit detection quantification calculated be 0.29and 0.99µg/mL, respectively. Accuracy precision described relative standard deviation recovery percentages. results show that CTAB-coated MNPs could employed extraction azo dye from different samples. successfully pharmaceutical forms spiked samples river water.